Composite

Part:BBa_K2187000:Design

Designed by: Ella Coleman   Group: iGEM16_Baltimore_BioCrew   (2016-10-14)


Lipase gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 634
    Illegal XhoI site found at 697
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a lacl regulated promoter, BBa_R0011, so that we could induce it to very high levels of expression. We also added a prefix and suffix, a BBa_B0030 RBS, and a BBa_B0015 terminator.


Source

We used the sequence of the genomic DNA:GAP38373.1 from GenBank.

References