Composite
Part:BBa_K2187000:Design
Designed by: Ella Coleman Group: iGEM16_Baltimore_BioCrew (2016-10-14)
Lipase gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 634
Illegal XhoI site found at 697 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added a lacl regulated promoter, BBa_R0011, so that we could induce it to very high levels of expression. We also added a prefix and suffix, a BBa_B0030 RBS, and a BBa_B0015 terminator.
Source
We used the sequence of the genomic DNA:GAP38373.1 from GenBank.